Setting the basis for transient DNA transformation and transformant selection in the red macroalga Gracilariopsis lemaneiformis

Gracilariopsis lemaneiformis ( Gp. ) is an economically important agar-producing red alga applicable in the food and cosmetic industries. The genetic knowledge of this species is, however, limited, tools for studying engineering it are lacking. This has limited understanding its developmental genetics hindered development new strains, developing would allow to tackle these problems. Here, transient DNA transformation via microparticle bombardment reported first time species, as well efficient exogenous gene expression driven by CaMV35S promoter, endogenous GlAct1 Pyropia yezoensis PyAct1 promoter transformed branches. Moreover, Blue Fluorescent Protein (BFP) demonstrated be a suitable reporter studies . Screening antibiotic sensitivity needed transformation, selection transformants also alga. Hygromycin B (Hyg) determined most effective selection. Hyg resistance shown confer at concentration 1 mg.ml -1 , but no individual could regenerated so far. These results promising future refining experimental conditions, instance, using different promoters techniques facilitating penetration cells.